$X$ is a subset of $Y$, written
\[X\subseteq Y,\]if
\[\forall x :\enspace x\in X \enspace\rimp\enspace x\in Y.\]$X$ is a proper subset of $Y$, written
\[X\subset Y,\]if
\[X\subseteq Y \enspace\land\enspace X\neq Y.\]
$\varnothing$ is the only set that is a subset of any non-empty set.
Proof.
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$\varnothing$ is a subset of any set since no $x\in\varnothing$.
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If $X$ is a subset of any non-empty set, then $X\subseteq\{X\}$. By ZF#PROP-SLF, $X\neq\{X\}$, so $X=\varnothing$.
For any $X$,
\[X \subseteq X.\]
For any $X$, $Y$ and $Z$,
\[X\subseteq Y \,\land\, Y\subseteq Z \enspace\rimp\enspace X\subseteq Z.\]
For any $X$ and $Y$,
\[X\subseteq Y \,\land\, Y\subseteq X \enspace\rimp\enspace X=Y.\]
Proof.By Axiom of Extensionality.
$\subseteq$ is a partial order.
Proof.By reflexivity, transitivity and antisymmetry of $\subseteq$.